Antibiotic resistance characteristics of environmental bacteria from an oxytetracycline production wastewater treatment plant and the receiving river.

نویسندگان

  • Dong Li
  • Tao Yu
  • Yu Zhang
  • Min Yang
  • Zhen Li
  • Miaomiao Liu
  • Rong Qi
چکیده

We characterized the bacterial populations in surface water receiving effluent from an oxytetracycline (OTC) production plant. Additional sampling sites included the receiving river water 5 km upstream and 20 km downstream from the discharge point. High levels of OTC were found in the wastewater (WW), and the antibiotic was still detectable in river water downstream (RWD), with undetectable levels in river water upstream (RWU). A total of 341 bacterial strains were isolated using nonselective media, with the majority being identified as Gammaproteobacteria. The MICs were determined for 10 antibiotics representing seven different classes of antibiotics, and the corresponding values were significantly higher for the WW and RWD isolates than for the RWU isolates. Almost all bacteria (97%) from the WW and RWD samples demonstrated multidrug-resistant (MDR) phenotypes, while in RWU samples, these were less frequent (28%). The WW and RWD isolates were analyzed for the presence of 23 tetracycline (tet) resistance genes. The majority of isolates (94.2% and 95.4% in WW and RWD, respectively) harbored the corresponding genes, with tet(A) being the most common (67.0%), followed by tet(W), tet(C), tet(J), tet(L), tet(D), tet(Y), and tet(K) (in the range between 21.0% and 40.6%). Class I integrons were detected in the majority of WW and RWD isolates (97.4% and 86.2%, respectively) but were not associated with the tet genes. We hypothesize that the strong selective pressure imposed by a high concentration of OTC contributes to the wide dissemination of tetracycline resistance genes and other antibiotic resistance genes, possibly through mobile genetic elements.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Tracking of chloramphenicol, erythromycin, and sulfamethoxazole antibiotic-resistant bacteria from untreated wastewater effluents to receiving river

Background: The aim of this study was to investigate the frequency and type of bacteria resistant to chloramphenicol (CHL), erythromycin (E), and sulfamethoxazole (SXT) antibiotics from untreated wastewater effluents to receiving river. Methods: In total, 32 samples were taken from eight sites located in the raw wastewater to the downstream of the receiving Ghotor river in Khoy city. Resistant...

متن کامل

Role of organic matter, clay, and iron contents in the sorption of oxytetracycline on river sediments

Background: Oxytetracycline (OTC) is a widely-used antibiotic; metabolism studies indicate only partial assimilation of it inside the body. Traditional wastewater treatment plants cannot remove OTC, and this results in the release of considerable amounts of the drug into aquatic environments. There is much concern over the role of residual antibiotics in the development of new generations of ba...

متن کامل

Bacterial community characteristics under long-term antibiotic selection pressures.

To investigate bacterial community characteristics under long-term antibiotic selection pressures, water samples from the upstream and the downstream sections of two rivers individually receiving the treated penicillin G and oxytetracycline production wastewater, as well as the anaerobic and the aerobic effluent of the penicillin G production wastewater treatment plant, were taken and analyzed....

متن کامل

Evaluation of Antibiotic Resistance Pattern of Pathogenic Bacteria in the Yasooj Municipal Wastewater Treatment Plant

Background & objectives: Wastewater treatment plants represent one of the most important approachs for water conservation. On the other hand, wastewaters can act as a source of antibiotics in aquatic environments. The aim of this study was to evaluate the performance of the Yasooj municipal wastewater treatment plant for removal of Escherichia coli, Pseudomonas aeruginosa and Listeria monocytog...

متن کامل

Urban wastewater effluent increases antibiotic resistance gene concentrations in a receiving northern European river.

Antibiotic-resistant bacteria are an emerging global problem that threatens to undermine important advances in modern medicine. The environment is likely to play an important role in the dissemination of antibiotic-resistance genes (ARGs) among both environmental and pathogenic bacteria. Wastewater treatment plants (WWTPs) accumulate both chemical and biological waste from the surrounding urban...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Applied and environmental microbiology

دوره 76 11  شماره 

صفحات  -

تاریخ انتشار 2010